In vitro Assessment of the Free Radical Scavenging Activity of Psidium Guajava

Observable significant revisit of ancient approach to prophylaxis and anaphylaxis (herbaltherapy), though with modern dimensions of study envelopes our world of research today. Reports on the medicinal use of parts of Psidium guajava (Myrtaceae), including leaves and stem barks have been reported in, and beyond Africa. Air dried leaves of P. guajava were powdered and extracted with 95%v/v methanol by maceration, and the extract concentrated at 40 C using Rotary evaporator. The weight of the o extracted plant material was recorded for yield calculations. In vitro assessment of the ability of the extract to scavenge the Reactive Oxygen Species (ROS), hydrogen peroxide, superoxide and the synthetic radical 1,1-Diphenyl-2-picrylhydrazyl (DPPH) was determined with reference to the synthetic antioxidant Buthylated hydroxyanisole (BHA). Plant extract showed concentrationdependent scavenging activity on all reactive species used. Scavenging activity of plant extract on hydrogen peroxide and superoxide was more than that of BHA on same. However, BHA showed greater DPPH scavenging activity than plant extract.